Journal: Cardiovascular Diabetology

Article Title: The sodium–glucose co-transporter 2 inhibitor empagliflozin attenuates cardiac fibrosis and improves ventricular hemodynamics in hypertensive heart failure rats

doi: 10.1186/s12933-019-0849-6

Figure Lengend Snippet: Empagliflozin attenuates hypertension-induced collagen deposition in cardiac fibrosis of left atrial and left ventricular. Collagen fiber deposition and arrangement was demonstrated by second-harmonic generation (SHG) microscopy for left atrial ( a ) and left ventricular ( b ) tissue of normo-tensive rats (WKY), spontaneous hypertensive rats (SHR), and empagliflozin-treated SHR (EMPA). All scans are transmural and show the whole myocardial wall (from left to right: endocardium or luminal surface to epicardium or pericardium). Collagen deposition is demonstrated with high SHG intensity

Article Snippet: The authors also thank Dr. Shean-Jen Chen for providing the use of the second-harmonic generation microscopy at College of Photonics, National Chiao Tung University, Tainan, Taiwan.

Techniques: Microscopy

Journal: Laboratory Animal Research

Article Title: Highlights on selected microscopy techniques to study zebrafish developmental biology

doi: 10.1186/s42826-020-00044-2

Figure Lengend Snippet: a A presentative diagram of imaging zebrafish embryo by Second Harmonic Generation Microscopy (SHG) and Third Harmonic Generation (THG): A titanium-sapphire laser generates pulses of 200 fs at 1200 nm. The laser beam passes on galvanometric mirrors (GM) to reach the sample through the objective lens. A dichroic mirror reflects the scattered signals from the sample, and the other dichroic mirror separates them into two channels (THG) and (SHG/2PF). The signals focused on the photomultiplier tube detectors (PMT), one for THG, and the other for SHG and 2PF. b A scheme of the light spectrum in SHG and THG microscopy. The excitation wavelength is 1200 nm, while the emission spectra of THG at 400 nm, SHG at 600 nm, and Two-Photon 2PF from 600 to 800 nm with a peak at ~ 650 nm generated by the sample

Article Snippet: Here, we briefly discuss the engagement of zebrafish in three models of advanced microscopy techniques: Two-Photon Microscopy (TPM), Second Harmonic Generation, (SHG), and Light-Sheet Microscopy (LSM).

Techniques: Imaging, Microscopy, Generated